把窗体的FormBorderStyle设为FormBorderStyle.None

using System.Runtime.InteropServices;

        private const uint WS_EX_LAYERED = 0x80000;
        private const int WS_EX_TRANSPARENT = 0x20;
        private const int GWL_STYLE = (-16);
        private const int GWL_EXSTYLE = (-20);
        private const int LWA_ALPHA = 0x2;

        [DllImport("user32", EntryPoint = "SetWindowLong")]
        private static extern uint SetWindowLong(
        IntPtr hwnd,
        int nIndex,
        uint dwNewLong
        );

        [DllImport("user32", EntryPoint = "GetWindowLong")]
        private static extern uint GetWindowLong(
        IntPtr hwnd,
        int nIndex
        );

        [DllImport("user32", EntryPoint = "SetLayeredWindowAttributes")]
        private static extern int SetLayeredWindowAttributes(
        IntPtr hwnd,
        int crKey,
        int bAlpha,
        int dwFlags
        );

        /// <summary>
        /// 使窗口有鼠标穿透功能
        /// </summary>
        public void CanPenetrate()
        {
            uint intExTemp = GetWindowLong(this.Handle, GWL_EXSTYLE);
            uint oldGWLEx = SetWindowLong(this.Handle, GWL_EXSTYLE, WS_EX_TRANSPARENT | WS_EX_LAYERED);
            SetLayeredWindowAttributes(this.Handle, 0, 100, LWA_ALPHA);
        } 

要时窗体恢复正常，只要执行以下语句：
                this.FormBorderStyle = FormBorderStyle.None;